ABSTRACT. Today, obtaining high-quality products based on scientific and technical achievements and introducing them to our economy is one of the most urgent tasks, is big.
Today, chemistry and biotechnological sciences cannot be imagined without chromatography. 70–80% of research in many fields, including organic chemistry, is contributed by chromatographic methods.
Therefore, the purpose of this science is to meet the requirements of the main compounds, proteins, peptides, carbohydrates, fats, vitamins, minerals and other compounds found in food products, modern chromatographic spectrophotometric and other compounds used in purification and other physico-chemical methods, consists of introducing the possibilities of styles.
Keywords. Chromotography, spectrometry, adsorption, chromatography precipitation, distribution.
Relevance of the topic. He learns modern chromotography , spectophometry and other physical chemical analysis techniques and their theoretical foundations. A chromotographic process, the chromotographic zone takes up methods to optimize fractionation conditions of ways to separate closely moving zones from each other.
Chromotography is a physicochemical method of analysis and research of VSA-they mixtures of separation-based substances that occur due to the distribution of components in the groove through the Phase Flow in motion from the excitable phase layer..
On the basis of chromotographic processes, there are many repeated sorption and dysorption processes, in which the components are excitable and their separation occurs at the expense of the difference in distribution coefficients between the phases in motion.
It will be extremely necessary for them to know modern and classic methods of analyzing the properties of matter. That is, the following;
1.Adsorption spectroscopy
2.Fluorescent spectoroscopy
3.Microwave spectoroscopy
4.Infrared spectoroscopy
5.Nuclear-magnetic resonance
6.Electron-paramagnetic resonance
7.Proton-magnetic resonance
8.Macc spectoroscopy
9.The basics of Fure-spectoroscopy.
Chromotographic methods are divided into three main types;
1.Adsorption,
2.Distribution,
3.Squat.
On the basis of adsorption chromotrography, adsorption of the secreted substances lies on the surface of the selected solid adsorbent.
On the basis of distribution chromotography lies the different solubility of the substances being separated in the liquid. The main condition for their separation is the difference in the solubility of substances.
On the basis of deposition chromotography, the separation lies in the formation of difficult soluble compounds as a result of the reaction between the substance and the precipitating reagent.
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